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Electrochemical Detection
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Blood Test for Lead Poisoning

Lead Care System Analysis for Blood Lead Measurements, Lead Analysis with Anodic Stripping Voltammetry, Reagents, Clinical Laboratory Analysis Service for Protoporphyrin, and Pediatric Blood Lead Poisoning

LeadCare system, a simple, foolproof way to perform blood lead measurements using a finger stick or venous sample. No more waiting days for expensive lab analyses. You get quantitative blood lead results equivalent to those reported by outside laboratories in just three minutes.

Lead Poisoning and Blood Lead Testing

The LeadCare System is for the determination of lead in whole blood. When you test young patients for lead levels, you want fast, accurate, inexpensive results. You want the A LeadCare system analysis costs far less than you'd pay an outside laboratory, and it qualifies for reimbursement as a quantitative blood lead. You'll also cut your staff's result-tracking and administrative time. You'll save your patients days of possibly needless worry plus the time-consuming inconvenience and cost of a return visit. Blood lead measurement couldn't be easier.

LeadCare is easy and safe to use. The hand-held analyzer is portable and requires neither manual calibration nor refrigeration. Its unique gold electrode sensor contains no mercury or other toxic materials. The point-of-care LeadCare system was developed by ESA and Andcare with a grant from the CDC. It's the diagnostic tool which makes sense medically and economically.

ESA has developed multiple solutions for the detection of Lead poisoning

  • LeadCare® Portable Blood Lead Analyzer
  • Model 3010B Clinical Blood Lead Analysis System
  • Laboratory Analysis Service

LeadCare® Childhood Blood Lead Testing

STEP ONE:  Draw a capillary or venous blood sample using EDTA or heparin as anticoagulants

STEP TWO: Using the pipette provided with the kit, dispense 50 µl, about two drops of blood, into the reagent and mix.

STEP THREE: Transfer it to the sensor strip. Press the button. Just three minutes later, you have your result.

Accuracy in blood lead testing

LeadCare System vs. Atomic Absorption Spectroscopy performed at a major lead outreach and referral clinic/hospital

Number of Samples: 112
Slope: 1.07
Intercept: -0.57 µg/dl
Correlation coefficient: 0.97 

Lead analysis

Lead analysis in your laboratory reduces turn-around time, cuts costs and generates revenue while providing a needed service for your community.

Anodic Stripping Voltammetry (ASV)

Using ASV and 30 years of experience, ESA makes blood lead analysis rapid and routine, without the high costs of other analytical methods. The system incorporates trace metal-free pre-packaged reagents and unique instrumentation.

The Instrument:  The Model 3010B consists of two components—sensing and electronics. The sensing unit includes a test electrode, a stirring assembly and two electrodes that carry current and regulate potential. This process is the heart of the ASV system. The electronics unit controls the analysis cycle, sorts the electronic signals and calculates the results.

Model 3010B Features

  • Rapid Analysis— only 90 second/sample
  • No Special Laboratory Setup—unlike AA and ICP/MS, no hoods, gases or flames are required
  • Small Footprint
  • Low Reagent Costs— only prepackaged reagents needed
  • Highly skilled operators not required
  • Reimbursable as a quantitative blood lead

The Reagents

Metexchange® kits include chemical pretreatment reagents, calibrators, controls and performance verification mixtures. The unique Metexchange pretreatment reagent eliminates the necessity of performing acid digestion.  Reagents come premeasured in easy to use snap cap vials.  All reagents are manufactured and available from one source — ESA

ESA Clinical Laboratory Services

ESA's Laboratory Analysis Service has been providing rapid and accurate blood lead and protoporphyrin analyses to the pediatric and industrial communities for more than 30 years.  ESA's Laboratory is a CLIA Certified, OSHA Approved, and Massachusetts Licensed Clinical Laboratory.  In 1972, when the DHEW, pre-CDC, contracted a laboratory to provide the Central Laboratory with "micro" blood lead sampling and analytical services to evaluate the extent of Pediatric Blood Lead Poisoning in the United States, ESA was that laboratory.   Since the beginning of the program, ESA has served, and continues to serve, as one of the Reference (Target-Value-Setting) Laboratories for the blood lead proficiency testing programs operated by the Commonwealth of Pennsylvania, and the CDC Center for Environmental Health (contracted to the University of Wisconsin for execution).

Blood Lead  ESA was the original reference lab for blood lead for the CDC and continues in that capacity today.  The laboratory is also OSHA approved and performs this service for industrially exposed populations.

CoEnzyme Q9 and Q10 in Plasma both the oxidized and reduced forms are measured by this method.

Fat Soluble Vitamins  Using ESA’s CoulArray® technology, serum or plasma is simultaneously analyzed for vitamin A (Retinol), b-Carotene, Lycopene, d-Tocopherol, CoEnzyme Q10, Retinyl Palmitate, Lutein, alpha-Tocopherol, and gamma-Tocopherol.  This assay has been used successfully by ESAL on the NIST Micronutrient Measurement Quality Assurance Program (M2QAP) Round Robin.

Plasma Catecholamines  ESAL’s method can detect low levels (20 pg/mL) of dopamine, epinephrine, and norepinephrine in sample volumes of less than 1 mL.

Plasma Metanephrines  ESA has received FDA approval for this clinical test.

Vitamin C  in Plasma ascorbic acid is measured in this assay.

Vitamin D3  25-Hydroxy Vitamin D3 (25-HOD3) is the primary circulating form of vitamin D in humans. The plasma level of 25-HOD3 is generally considered to be the most reliable index of Vitamin D status.  This method has been used for clinical trials.

Vitamin K1  Phylloquinone is an important fat soluble vitamin involved in coagulation.  This assay for K1 in plasma has been utilized in clinical trials.

Total CoEnzyme Q10  CoEnzyme Q10 has always been part of the Vitascan® assay.  This new simplified assay measures total CoQ10.

Blood Lead Analyzer

Blood Lead Analysis
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